Retroviral Vector
The MMLV retroviral gene expression vector system is a highly efficient system that stably integrates foreign genes into mammalian cell genomes, and is also a particularly popular gene transduction method in iPS cells preparation.
The MMLV retroviral vector is derived from Moloni (A's) murine leukemia virus and belongs to the retroviral family, and the wild-type retroviral genome is a linear double plus-stranded RNA.
After the recombinant retroviral vector of MMLV was constructed, it was transfected into packaging cells together with auxiliary plasmids. In the packaging cell, the DNA segment located between two long terminal repeats (LTRs) is transcribed into RNA, which is packaged into viral particles by viral proteins expressed by helper plasmids. The packaged live virus will be released into the supernatant, which can be directly collected or further concentrated to transfect the target cells.
When viruses transduce target cells, the viral RNA released into the host cell is reverse transcribed into DNA with the help of reverse transcriptase, which is then randomly integrated into the genome of the host cell. In the viral vector, the DNA fragments located in both LTR and the viral genome are stably integrated into the genome of the target cell.
For more information on retroviral gene expression vectors, please refer to the following literature.
The MMLV retroviral vector is derived from Moloni (A's) murine leukemia virus and belongs to the retroviral family, and the wild-type retroviral genome is a linear double plus-stranded RNA.
After the recombinant retroviral vector of MMLV was constructed, it was transfected into packaging cells together with auxiliary plasmids. In the packaging cell, the DNA segment located between two long terminal repeats (LTRs) is transcribed into RNA, which is packaged into viral particles by viral proteins expressed by helper plasmids. The packaged live virus will be released into the supernatant, which can be directly collected or further concentrated to transfect the target cells.
When viruses transduce target cells, the viral RNA released into the host cell is reverse transcribed into DNA with the help of reverse transcriptase, which is then randomly integrated into the genome of the host cell. In the viral vector, the DNA fragments located in both LTR and the viral genome are stably integrated into the genome of the target cell.
For more information on retroviral gene expression vectors, please refer to the following literature.
| Reference Documentation | Theme |
|---|---|
| Exp Hematol. 31:1007 (2003) | Review |
| J Virol. 61:1639 (1987) | Extended packaging signal increases the titer of MMLV vectors |
| Gene Ther. 7:1063 (2000) | Tropism of MMLV vectors depends on packaging cell lines |
| Nat Protoc. 6:346 (2011) | Tropism of MMLV vectors depends on packaging plasmids |
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